Phospholipids of Nocardia coeliaca.
نویسندگان
چکیده
The lipids of Nocardia coeliaca were separated into at least 10 components by the use of thin-layer chromatography. Phosphatidylcholine was the most abundant phospholipid in this organism, accounting for 25 to 40% of the total phospholipids. The major fatty acid components of the phosphatidylcholine were 14-methyl-pentadecanoic acid (41%), the other C(15) and C(17) iso- and anteiso-fatty acids (29%), and palmitic acid (13.5%). The next most abundant phospholipid was phosphatidylethanolamine (25 to 30%), followed by phosphatidylinositol (11 to 14%) and cardiolipin (7 to 15%). Phosphatidylethanolamine and phosphatidylinositol were very similar to the phosphatidylcholine in fatty acid composition, whereas cardiolipin was characterized by a higher content of palmitic acid (30%). In all of the phospholipids examined, only trace amounts of monounsaturated fatty acids were present. When washed cells of N. coeliaca were incubated with methionine-methyl-(14)C for 1 to 3 hr, the radioactivity was mainly incorporated into the choline moiety of the phosphatidylcholine. In contrast, acetate-1-(14)C or glycerol-1-(14)C was incorporated much more slowly into the phosphatidylcholine than into the other phospholipids and neutral lipids. No phosphatidylcholine was detected in 10 other species of Nocardia examined.
منابع مشابه
Comparison of Three Methods of Isolation of the Genus Nocardia from the Soil of Hospitals in Isfahan Province, Iran, and its Identification Based on Phenotypic and Molecular Methods
Background & Aims: Nocardia are gram-positive, aerobic, relative acid-fast, and opportunistic bacteria, and one of the causes of systematic infection around the world. The natural habitats of these bacteria are soil and dust. Hospitalized patients with immune deficiency are at high risk of transmission of opportunistic infection. Due to these facts and the complexities that exist in the isolati...
متن کاملComparison of Three Methods of Isolation of the Genus Nocardia from the Soil of Hospitals in Isfahan Province, Iran, and its Identification Based on Phenotypic and Molecular Methods
Background & Aims: Nocardia are gram-positive, aerobic, relative acid-fast, and opportunistic bacteria, and one of the causes of systematic infection around the world. The natural habitats of these bacteria are soil and dust. Hospitalized patients with immune deficiency are at high risk of transmission of opportunistic infection. Due to these facts and...
متن کاملDetection of Nocardia Asteroides Complex in Clinical Isolates by Real-Time Polymerase Chain Reaction
Background and Aims: Nocardia asteroides complex is the most common cause of infectious diseases due to nocardiosis. Interspecies differentiation of Nocardia genera is essential for prognosis and timely proper treatment, as well as for epidemiological studies. Since each genus has its own antibiotic resistance, precise careful diagnosis is of prime importance. As compared to biochemical and phe...
متن کاملFirst Report of the Isolation of Nocardia thailandica from the Bronchoalveolar Lavage of a Patient in Iran
Nocardiae are Gram-positive, filamentous, aerobic, relatively slow-growing, and weakly acid-fast bacteria which cause nocardiosis in humans. We describe a 53-year-old patient with chronic bronchitis referred to Al-Zahra Hospital, Isfahan. A bronchial washing sample was taken from the patient. A Nocardia-like microorganism was detected in microscopic evaluation. Based on the phenotypic and 16S r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 98 1 شماره
صفحات -
تاریخ انتشار 1969